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Cell imaging techniques in particular yield large-scale systematic image data characterizing gene and protein localizations [ 1].
Methods for realtime PCR, Western blotting, measurement of nitrite release, cell imaging techniques including morphometry apoptosis detection immunofluorescence and image acquisition and processing are described in the Supporting Information.
Advances in high-speed live cell imaging techniques, taking advantage of developments in the area of fluorescent probe design hold considerable promise for the investigation of the dynamic signaling processes governing platelet activation and function, both physiologically and pathologically.
This finding highlights the importance of live cell imaging techniques with nanoscale sensitivity or cryo-fixation in the interrogation of cellular structure, to complement more traditional chemical fixation methods.
Complementary live cell imaging techniques using the living cell as both a visual and molecular test tube is vital to our understanding of the dynamics and mechanisms underlying nuclear organization.
The signalling and transcriptional protein complexes are analyzed with mass spectrometry and cell imaging techniques to build a model of T cell activation and differentiation.
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Therefore, we applied live cell imaging technique based on MitoTracker green (Molecular Probes, USA) and concomitant Hoechst 33342 (Molecular Probes, USA) staining.
Here, Nicholson et al. used live-cell imaging techniques to analyze healthy human cells and cancer cells that had either the normal forty-six chrorosomes, or a defined extra chromosome.
Experiments tracking fluorescently tagged proteins through live-cell imaging techniques as well as the use of nuclear import assays in permeabilised cells should be routinely used in order to confirm the possible nuclear translocation of these proteins.
To directly track bulge SCs and study early steps of HF SC contribution for regeneration of the SG in more detail, a novel live cell imaging technique was developed.
The use of these NP-TGT molecules in combination with the high resolution high speed live cell imaging technique through TIRFM allows us to investigate several intriguing questions: (1) Does the BCR itself possess any mechanosensing properties?
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