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For life cell imaging cells were grown in glass bottom dishes in DMEM+ lacking phenol red.
For live cell imaging cells were grown in the same media but buffered with 25 mM Hepes pH 7 [20].
For live cell imaging cells were maintained as per the culture conditions and stained with either mitochondria specific dyes or with mitochondrial targeted GFP expression proteins.
For live cell imaging cells were plated on glass bottomed 30 mm dishes (Matek) and following wash-out of mevastatin were imaged using the 488 nm laser-line of a Zeiss LSM-510 inverted confocal microscope with 100× 1.4Napochromatat oil-immersion objective lens.
For live cell imaging, cells were seeded on glass, treated with CT20p, and stained with 1 uM JC-1, and imaged on the Ultraview spinning disk confocal microscope (PerkinElmer) as described above.
For MR cell imaging, cells need to be labeled with magnetic contrast agent to distinguish them from the surrounding tissues by MRI.
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For live cells imaging, cells were adhered with Poly-lysine on small glass bottom Petri dishes (MaTek).
For live-cell imaging, cells in chambers were imaged on a live cell station.
For live-cell imaging, cells were continuously observed using a PerkinElmer UltraVIEW VoX-3D Live Cell Imaging System (Shanghai, China) from 24 to 72 h post-transfection.
For live-cell imaging, cells were grown in multiwell plates, and treated with Zn2+ or Ni2+ and then loaded with zinquin as described above.
For live-cell imaging, cells were transferred into phenol red free L-15 medium supplemented with 10% FBS and kept on the microscope stage at 35°C during observation.
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