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Cell-associated radioactivity was detected after Cell harvesting (TomTec) on filtermats using a Betaplate Counter (MicroBeta TriLux, PerkinElmer).
After cell harvesting, the cells were tracked for 24 48 h.
Etoposide (Sigma-Aldrich) was included as a positive control (100 µM, exposure 4 h before cell harvesting).
To derepress the transcription of lac operon, 0.5 mm IPTG was added and cells were cultured for further 1 h before cell harvesting.
The dilute conditions under which microalgae suspensions are cultured have negatively impacted economical routes for cell harvesting using conventional approaches.
These systems circumvent the costly step of cell harvesting, whilst the product is extracted and prepared for downstream processing.
Glucose-1-phosphate was found to be formed in relatively high amounts after cell harvesting if enzymes were not inactivated.
Cell harvesting was conducted with a net recovery of solids and water higher than 95% with a solids concentration factor about 20 30.
Therefore, in vitro binding studies on human NET membranes were performed in a cell harvesting protocol.
Just one population over the 8 tested allowed a suitable cell harvesting for mRNA extraction.
Guidelines related to each of the cell harvesting procedures are described, and a discussion of the expected populations of cells harvested from these various sites is included.
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