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Molecular expression and cell function analysis were performed to evaluate the effect of EMT or MET on tumour-initiating capacity and other biological characteristics.
Results from Phase III trials, 34– 41 in line with previous preclinical and Phase II data, indirectly support a beneficial effect of liraglutide treatment on beta cell function, as measured by homeostasis model assessment for beta cell function analysis (HOMA-B), and by the proinsulin to insulin ratio.
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Besides the identification of previously studied genes and pathways involved in dendritic cell function, our analysis also revealed many novel candidate genes, which are likely important for dendritic cell differentiation and response to stimuli.
Insulin sensitivity was assessed by the glucose clamp technique and β-cell function by analysis of the glucose and C-peptide curves during the oral glucose tolerance test (OGTT) (75 g).
To address these questions and further elucidate the factors that mediate stem cell function, we undertook an analysis to identify genes whose expression is correlated to Oct4.
This new technology exhibits utility for the preparation of cell sources required for cell transplantation as well as microglial function analysis.
Although YAP gain of function led quite expectedly to CMZ cell overproliferation, the loss of function analysis revealed a more complex phenotype.
The process of harvesting human cells from patients with acute myocardial infarction, analysis of cell function, radiolabelling of monocyte subfractions, administration of cells into mice and finally performing imaging with either planar and tomographic (single-photon emission computed tomography (SPECT)) scintigraphy or positron emission tomography (PET) is a long process.
There is no doubt that future research will progress in the direction of more detailed analysis of cell function and precise manipulation of cell response with expanding sets of complex nanoscale tools.
At the present time, the analysis of cell function within arrays or on micropatterned surfaces is largely limited to intracellular markers, 10, 22, 27, 31 however, it is advantageous to be able to monitor secreted products in cell arrays.
We compared cells from patients ('patient cells') and those from healthy controls ('control cells') using flow cytometry, gene expression profiling, protein expression analysis and cell function analyses that included assays of cell proliferation, metabolic function and cell morphometry to quantify content and distribution of intracellular organelles.
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