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Cell fractions were isolated and examined by Western blotting.
The cell fractions were isolated as described in MATERIALS AND METHODS.
Data are expressed as percentage of radioactivity in the cell fractions.
All cell fractions led to engraftment indicating LIC activity of all leukemia cells.
Contents and gene expression of glucose transporter-2 (GLUT2) in cell fractions were examined.
The cell fractions were analyzed with respect to morphology (transmission electron microscopy) and function (phagocytosis).
Luciferase activity was determined from both supernatant and cell fractions.
In spite of these similarities, the two cell fractions displayed fundamental functional differences.
Cell fractions were then recovered and spun at 1150 rpm for 12 min at 4°C.
Whole cell fractions were prepared in a similar manner but the centrifugation step was omitted.
Both cell fractions underwent spontaneous apoptosis and were rescued by incubation with rCD40L (Supplemental Fig. 1B).
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