Thus, we used cell fractionation to prepare nuclear and cytoplasmic fractions from DU145 cells.
Membrane and cytosolic fractions were isolated using the QProteome cell fractionation kit (Qiagen; Crawley, United Kingdom).
Cell fractionation and immunoblot analyses supported these results (Fig. 5c and Supplementary Figs. 10 and 11).
Suzuki, K., Bose, P., Leong-Quong, R. Y., Fujita, D. J. & Riabowol, K. REAP: a two minute cell fractionation method.
Based on these results, the effect of PTEN on DAXX cellular compartmentalization was investigated using cell fractionation.
Cell fractionation studies showed that NF90 and its heteromeric partner, NF45, are predominantly nuclear and largely chromatin-associated.
Therefore, we analyzed the subcellular localization of lncRNAs in A10 cells, a cell line derived from vascular smooth muscle, through cell fractionation and transcript measurement.
Then, the cytoplasmic and nuclear protein were isolated by using the Cell Fractionation kit (BioVison, SF, USA) according to the supplier's instruction67.
The localization of l-asparaginase was carried out using cell fractionation techniques.
Cell fractionation experiments indicated that the Zifs enhanced nuclear localization of the Zif p53 DBD chimera.
Cell fractionation included isolation of the cytoplasm, periplasm, membrane, outer membrane vesicles, and extracellular media.
