Altogether, these results demonstrate that cells forming cells derived from MGNT exhibit a common and stable surface antigen and proteomic profile, as well as some features of stem/progenitor cells in long-term cultures.
RhoB-depleted DU145 cells had a significant reduction in the number of cells forming cell-cell contacts with each other.
In order to characterize the nature of the presumable 'cannibal' and 'prey' cells forming cell-in-cells, we performed immunohistochemical epithelial membrane antigen (EMA) and AE1E3 staining that confirmed their epithelial origin (Fig 2A and B).
But remarkably, when these immature cells were transplanted into mice, mature beta cells formed — cells that could maintain normal glucose levels after ablation of the mouse's intrinsic pancreatic beta cells (Kroon et al., 2008).
When grown on collagen/fibronectin gel support at an air liquid interface, airway epithelial cells formed cell layers morphologically resembling those in vivo.
Much like their relatives derived from bone marrow aspirates and skin fibroblasts [24], Muse-AT cells form cell clusters similar to ES cells when grown in suspension.
Migrating single cells formed cell-cell attachments, which led to formation of small aggregates.
DU145 and MCF10A cells formed cell-cell contacts and expressed both E- and P-cadherin, which localized to cell-cell contacts (Fig. 1A, B).
Remarkably, GFP-Akata cells formed cell-in-cell structures in higher frequency compared with uninfected parent AK31 cells in parallel (Supplementary information, Figure S1A).
Figure 6B shows that MLL cells form cell sheets that are significantly larger than either MLL-X5C2 or MLL-X5C5 cells (ANOVA, p < 0.0001, Tukey's MCT, p < 0.001).
