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The value propositions of femtocells are the low upfront cost to the service provider, increased system capacity due to smaller cell footprint at reduced interference, and the prolonged handset battery life with lower transmission power.
The cell position was defined as the center of mass of the cell footprint.
The appearance of individual sparklets when capsaicin was added became difficult to discern over time as the fluorescence across the cell footprint increased.
To quantify this, we calculated the coefficient of variation (CV) of the Ca2+ signal in the entire cell footprint; a greater spatial heterogeneity will produce a higher CV.
Cell footprint area, the [Ca2+]pm patterns in 3 and 20 mM glucose, and glutamate responsiveness were therefore always used to discriminate between α- and β-cells.
For measurements of Ca2+ influx, the standard deviation and mean fluorescence were measured for the entire cell footprint, which was determined from the basal fluorescence of the Oregon Green 6F dye infusion.
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The obtained cell footprints and boundaries were stored as pixelated information.
Interestingly, we could show in vitro that CCN3 is sequestered in cell footprints representing a secreted extracellular matrix.
Prominent cytoplasmic staining was observed in G59/540, as well as the cell footprints of G59/540 and H295R were strongly labeled.
Cell footprints can be deposited by various cell types in different arrangements and consists of extracellular matrix, including a variety of basement membrane proteins [ 30- 32].
Interestingly, since cells grown on coverslips and fixed in paraformadehyde tend to slough, we did note the presence of positively staining cell footprints, suggesting deposition of CCN3 protein in a secretable extracellular matrix.
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