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By reducing the volume of fluid in the sample application lines and the flow cell, flushing requirements to achieve clean baselines and minimum sample volumes are reduced.
Fluids carry nutrients to your cells, flush bacteria from your bladder, and prevent constipation.
Maximum extractions over 52 weeks were: 58% U, 50% Th, 36% Y and 45% of the REEs; observed in cells flushed fortnightly with a ferric sulphate lixiviant (0.5 g/l, pH 3.5).
Peripheral blood cytosmears and bone marrow cytospins (bone marrow cells flushed with a 21-gauge needle in 2% FBS in PBS) were stained with Wright's stain and examined by a hematologist (GS).
Extraction of total RNA from rat heart tissue, bone marrow cells flushed from femurs, purified myocytes, MDC, and MDC spheres, and one-step RT-PCR were carried out with commercially available kits (Qiagen, Valencia, CA).
Cells flushed from femurs and tibias were plated in RPMI medium (Sigma-Aldrich) with 10% FBS (HiClone), 100 U/ml penicillin, 100 µg/ml streptomycin (Invitrogen Life Technologies), nonessential amino acids (0.1 mM), sodium pyruvate (1 mM), 2-mercaptoethanol (50 muM), L-glutamine (2 mM) and 1% GM-CSF containing supernatant from the X-63Ag8-GM-CSF-transfected cell line [45].
Two hours later, mice were killed, BM (containing >90% MM cells) flushed and washed with PBS.
Cells flushed from the hollow fibers were washed with PBS, and then cells were lysised with ultrasonic cell crasher.
BM cells flushed from tibias and femurs were subjected to ammonium-chloride potassium red cell lysis before staining with antibodies.
Both femurs of each mouse were dissected and bone marrow cells flushed gently with 0.56% KCl solution into a centrifuge tube.
The presence of MCPyV DNA in the cellular pellet was probably caused by MCPyV- positive urogenital cells flushed into the urine.
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