Exact(1)
A cell fixing and permeabilisation kit (AbD Serotec, Oxford, UK) was used according to manufacturer's instructions.
Similar(59)
Upper panel, fixed cell fixed 1 hr post-fusion.
Figure 4 CSLM images of mouse fibroblast cells fixed on N + -bombarded MWCNTs.
In these experiments, we used S. solfataricus cells, fixed with 100%% methanol.
Erythrocytes were lysed and cells fixed with FACSlyse (BD Biosciences).
The HMDS was allowed to evaporate, leaving the cells fixed to aluminium scanning electron microscope stubs.
Immunofluorescence staining was performed on the infected and uninfected cells fixed at 2 and 48 hrs.
Cells fixed and stained with phosho-Rb antibodies were imaged using identical settings.
Hsp/c70 was detected by confocal microscopy in cells fixed in 4% formaldehyde.
Defined media were changed every 3 days and cells fixed in 4% paraformaldehyde after 21 days.
Cells fixed with 2% formaldehyde were stained for β-Gal using standard procedures.
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