Indeed, in a homogeneous tumor, migration reduces cancer cell fitness (Chen et al. 2011).
Importantly, SGPA enables quantification of phenotypes that are not readily detectable in ordinary genetic analysis of cell fitness.
Prevention of medial calcification in patients with chronic kidney disease requires the maintenance of vascular smooth muscle cell fitness.
Misfolded proteins tend to form cytotoxic intracellular aggregates, decreasing cell fitness and leading to degenerative disorders in humans.
Sufficient supply of glutamine and its utilization by T cells has an indispensable role for the development of TEFF cell fitness [20, 21].
Three of the four single mutants had no apparent effect on cell fitness at growth temperatures ranging from 23°C to 37°C (Fig. 1B, top panel).
We need to learn how to attack the cancer and at the same time support the immune system, boost normal cell fitness, and restore a more normal niche.
Here, we address this issue by coupling cell fitness to the production of thiamine diphosphate in Escherichia coli using a synthetic RNA biosensor.
We use this strategy to interrogate a library of transposon mutants and elucidate the native gene network influencing both cell fitness and thiamine production.
In 3D cell cultures, cell fitness is maintained, the physiological cells-cells and cell-matrix interactions are restored and an extracellular matrix surrounds the cells.
Production of chemicals in microbes often employs potent biosynthetic enzymes, which can interact with the microbial native metabolism to affect cell fitness and product yield.
