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In summary, the confocal images show a lower SNR and SBR for an atomic vapor cell filter compared to a commercial filter.
The digestion solution was filtered with a cell filter (pore size 70 μM; BD Bioscience).
The spores were then washed by liquid YPG medium and filtered by cell filter to get rid of the hyphae.
After digestion, the suspended cells were filtered through a 70 μm cell filter to avoid large cell aggregates.
To prepare donor lymphocytes, spleen and mesenterial lymph nodes of C57BL/6 mice were removed and pressed through a cell filter (40 μm pore size).
Mouse brains and spinal cords were isolated, homogenized in RPMI medium containing 0.5 units/ml Wünsch Liberase RI (Roche Applied Science) and 14 g/ml DNase I (Roche Applied Science) and passed through a 100-µl cell filter.
Similar(46)
The data passed the quality control and cell filtering processes described in the ref. 14.
Filtration is carried out in gravity filters (screens, dewatering bins), in centrifugal filters (screen centrifuges), in vacuum filters (drum cell filters, disk filters), or in pressure filters (filter presses).
In the ODEP microfluidic system, a four-cascade cell isolation using four optical light-based virtual cell filters was designed.
The volume of the concentrated samples was further reduced to 30 µL using a 0.5 mL 10 kDa cut-off Ultra Cell filter-unit using a Microfuge 18 tabletop centrifuge (Beckman Coulter) at 14,000 rpm.
Four mL of cell media were concentrated to 50 µL using 10 kDa cut-off Ultra Cell filter-unit (Millipore) by 15 min centrifugation at 4,000×g using a centrifuge (Sigma 4K10) equipped with a swing-out rotor.
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