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Protein extracts were obtained using Cell Extraction Buffer buffer (BioSource International, CA, USA) plus protease inhibitor cocktail (Sigma).
Proteins were extracted using a Whole Cell Extraction Kit (EMD Millipore Corporation, Billerica, MA, USA), according to the instructions.
Nuclear extracts were prepared as previously described using a cell extraction kit (Active Motif, Carlsbad, CA, USA).
Protein extracts were normalized to wet weight in an appropriate volume of cell extraction buffer.
He described pepper spray and forcible cell extraction.
It was only the third "cell extraction," as they are called, since l982.
One of the three officers had no training in immediate response or emergency cell extraction.
Two samples from the same period are needed, one for infectious-disease testing and one for cell extraction.
One of Dhiab's attorneys, Jon Eisenberg, said the government possess thousands of tapes detailing feeding and cell extraction conditions of the other detainees.
Volzer said he has still not been allowed to see a video of the cell extraction that was recorded by a deputy.
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Whole-cell extraction was performed with M-PER Reagents (Pierce Biotechnology, Rockford, IL, USA) according to the manufacturer's instructions.
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