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The cell extract was then centrifuged at 14,000g.
Cells were grown for 48 hours and then harvested for cell extract preparation.
Filipowicz, W. & Shatkin, A. J. Origin of splice junction phosphate in tRNAs processed by HeLa cell extract.
The DhaB and KGSADH activities in the crude cell extract were measured using previously reported methods20,72.
Cell extract (10 μg/mL) of L. acidophilus GP1B exhibited the highest inhibitory activity among 5 to 40 μg/mL cell-extract concentrations.
The obtained labeled cell extract contained the whole yeast metabolome.
A combination of algae, phyto stem cell extract, and peptides helps rejuvenate skin and diminish signs of stress.
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Fifty microlitre of lysate was reserved as whole-cell extract input control.
Log-phase cells were exposed to phleomycin for indicated times and whole-cell extract was prepared (see Methods).
Exponentially growing yeast cells were exposed to phleomycin for indicated times and whole-cell extract was prepared.
A whole-cell extract was prepared as previously described [ 24].
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