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Gqα (GNAQ), G11α (G14α1), G14α (GNand) and G15α (GNA15) each have very different tissue and cell expression patterns.
We demonstrate how the bistable nature of the lac operon's feedback, when perturbed by patterning lactose (inducer) and glucose (inhibitor), can lead to coordination of cell expression patterns across a field in ways that mimic motifs seen in developmental biology.
To obtain a global overview of stem cell expression patterns, we assembled a compendium of data from 30 different studies assaying gene expression of 49 stem cell populations representing twelve different types of stem cells including hematopoietic, retinal, neural, embryonic, and intestinal (Figure 1; Table 1).
We were surprised by the observation that the bistable nature of the lac operon's feedback system, when perturbed by patterns of lactose (inducer) and glucose (inhibitor), can lead to coordination of cell expression patterns across a field in ways that mimic motifs seen in developmental biology.
We next performed whole-mount fluorescent in situ hybridization (FISH) to determine whether the candidate genes exhibiting discrete mesenchymal cell expression patterns overlapped with AGAT-1 expression.
Because of the importance of senescence, in the present study we utilize laser capture microdissection (LCM) to specifically harvest senescent cells from the annulus, determine gene expression patterns using microarray analysis, and then compare and contrast the senescent cell expression patterns with patterns from paired non-senescent cells harvested from the same histologic section.
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zpmca2 was expressed with a housekeeping pattern, while zpmca1a showed a hair cell expression pattern during embryonic stages (data not shown).
To address the transgene cell expression pattern, EGFP immunohistochemical localization on both infant and adult quadriceps was then investigated.
The reaction of the immune cell to neurotransmitters is variable depending on the context of receptor engagement (activation state of the cell, expression pattern of neurotransmitter receptors, microenvironment, cytokine milieu, and distance from the catecholamine source (concentration)).
This experimentally validated network topology generated three stable states, one of which was composed of 32 interconnected internal states, including the one that matched the stem cell expression pattern.
T-cell expression patterns of the α4 and β1 integrin chains were investigated as putative T-cell lung homing markers (Fig. 8).
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