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Immunoblot analysis using α-MakA antibody showed that, despite its strong whole cell expression level (Fig. 3f, lane 4, upper panel), MakA-mGFP was almost undectable in the supernatant while secretion of native MakA was still detected although at a reduced level (Fig. 3f, lane 4, lower panel).
After induction of high level expression of MakA-Top7-mGFP in V. cholerae strain A1552, we did not detect secretion of the chimeric protein and we observed much reduced levels of secretion of native MakA, although the whole cell expression level was not changed (Fig. 3f, lane 5 upper and lower panels), implying an entrapment of the chimeric protein in the narrow central channel of flagellum.
To objectively quantify the per cell expression level of each receptor, fluorescein isothiocyanate (FITC) and phycoerythrin (PE).
(E- G ) StE- G Steadyphostateylation of EGFR at the phosphorylationatiofs displayEGFRn (B – D ) upon vatying the total amount of EGFR PMr cell (expression level).
Understanding how cell expression level might impact on disease resistance is complicated in humans due to the presence of three class I loci, so we began by studying a simpler animal system before examining human class I alleles.
The whole cell expression level of LipA was almost the same in the three expression plasmid systems E. coli BL21/pET28a- lipAB, E. coli BL21/pETDuet- lipA lipB and E. coli BL21/pACYCDuet- lipA lipB in the same condition.
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Importantly, high cancer cell expression levels of podoplanin correlated with lymph node metastasis and reduced survival times in a large cohort of 252 oral squamous cell carcinoma patients.
Gene expression in estrogen-treated cells is moderately higher at 24 than 12 hours, but there is a comparable increase in the untreated cell expression levels.
This cell line displays normal cell expression levels of EGFR (2 × 10 EGFR/cell).
Clusters with pronounced CD4+ T cell expression levels (e.g. Figure 5) were then selected for functional analysis with GO.
After probe-to-gene mapping, relative CD4+ T cell expression levels were compared for different sets of human genes.
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