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Our cell expression assay being devoid of OBPs suggests that AaOR8 is sufficient to account for enantioselectivity and is consistent with electrophysiological data gathered from the mosquito Cx. quinquefasciatus [33].
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Data for the hypodermal and head cell expression assays come from two and three independent experiments, respectively.
Furthermore, we showed that single-cell expression assays coupled with time-lapse microscopy can resolve the identity and the lineage relationships between progenies of single cultured CPCs.
Our previous work, using RNA-FISH to conduct single-cell expression assays in tammar wallaby fibroblast nuclei, provided a similar result to this study where all loci tested escaped XCI to some degree [ 39].
Each of these mutant constructs was faithfully expressed in the 293-cell transient expression assay (see Fig. S2).
Here, we focus on the reverse-transcriptase qPCR (rt-qPCR -based Fluidigm (San Frt-qPCR -basedsingle-cell gene exprt-qPCR -based which provides simultaneous measurements oFluidigm96 geneSan Francisco, CA as minute as a single cell.
A structure activity relationship study was conducted using cell-surface expression assay to quantify folding/trafficking efficiency of P23H rhodopsin.
For single-cell gene expression assays, cells were sorted into individual wells of 96-well PCR plates.
For single-cell gene expression assays, cells were sorted into individual wells of 96-well PCR plates using the modified plate holder illustrated in Figure 1.
Western blotting, cell surface expression assays were as described previously (Roberts and Evans, 2004).
To gain deeper insight into the gene expression program of murine HSCs, we combined single-cell functional assays with flow cytometric index sorting and single-cell gene expression assays.
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