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Diffusion cell experiments performed with α-pinene using inactivated biofilm, previously grown on methanol and α-pinene, immobilized in agar indicate that initially sorption takes place within the film but after this initial lag phase, the transport rate is not significantly different from agar indicating the ratio of the diffusion and partition coefficient of the mobile phase is the same.
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The details of the cell culture routine used, cell motility experiments performed and the bimodal analysis technique are elaborated elsewhere [31].
For cell viability experiments performed under hypoxic conditions, cells were cultured in a hypoxia chamber as described above.
XZ conducted the cell culture experiments, performed PCR analyses, immunoblot analyses, uptake experiments and statistical analyses and wrote the manuscript.
Cholecystokinin and oligopeptides elicited increases in intracellular calcium in single-cell imaging experiments performed using cultured D-cells.
In the case of biofilm formation we observed the same lack of aggregation for ΔXacFhaB cells in experiments performed in SB or XVM2.
MJV performed the tumor cell experiments, MFM performed the NG experiments.
These methods include details on the cell lines used, RNAi knockdown experiments, and other cell biology type experiments performed here.
Constructs were transfected into HEK293 cells and immunoprecipitation experiments performed as described previously (50).
During membrane process design, flat sheet test cell experiments are performed to determine intrinsic membrane properties.
All cell experiments were performed in accordance with protocols approved by the Biosafety Committee of the Molecular Sciences Research Center, University of Puerto Rico, USA.
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