Table 1 Patient cohort, clinical information, and cell enumeration.
The clinical community has recently defined the need for stem cell enumeration kits that incorporate viability dyes.
Single-platform flow cytometry assays offer the significant advantages of speed and reproducibility, and have therefore become the gold standard in stem cell enumeration.
The purpose of this study was to evaluate a novel assay, BD Biosciences' (BD) stem cell enumeration kit (SCE kit‡), in relation to Beckman Coulter's (BC) commercially available BC Stem-Kit™.
Multi-site evaluation of the BD Stem Cell Enumeration Kit for CD34 cell enumeration on the BD FACSCanto II and BD FACSCalibur flow cytometers.
During the cell enumeration procedure, it was noted that the cells at day 7 appeared smaller than at day zero.
Purified DCs samples contained >95% CD1c+ DCs as evaluated by the Blood Dendritic Cell Enumeration Kit (Miltenyi Biotech).
After the incubation period for biofilm formation on the glass surfaces, unattached or loosely attached cells were removed by washing with N2 purged distilled water, prior to biofilm cell enumeration.
In the final 77 patients, EPCs were also identified on the basis of cell surface marker expression (CD133, CD34, and vascular endothelial growth factor receptor-2 [VEGFR-2]) and aldehyde dehydrogenase (ALDH) activity.Endothelial progenitor cell enumeration based on fluorescence activated cell sorting was more precise than culture assays.
However, its use may be impaired because soybean seeds under actual field growing conditions are obviously non-sterile and the bacterial or fungal seed microbiota normally interferes with the counting, which implies that bradyrhizobia cell enumeration is normally more difficult starting from non-sterile seeds than from inoculants during quality control procedures (Hiltbold et al., 1980).
Cell enumeration and viability were assessed by use of a hemocytometer and Trypan blue exclusion.
