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In the case of the spectrophotometer, the instrument was operated in double beam mode with the reference cell empty during sample acquisition.
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Dolly the sheep, the first mammal to be cloned, was created by inserting DNA from the nucleus of a sheep mammary gland cell into an egg cell emptied of its own nuclear DNA.
With swingeing budget cuts and chronic overcrowding, prisons can no longer afford to keep cells empty, sometimes for several weeks at a time, on the off-chance they will be called on to take problem inmates.
We injected the three types of H460 cells (wild cells, empty vector cells, and NPC2-shRNA-containing cells) into nude mice and examined xenografts for papillae formation.
The nuclei of the basal cells (empty arrows) were also relatively weakly stained regardless of its distribution along the whole epididymis.
Legend for table cells: empty = no statistical significance; * = p<0,01; ** = p<0,001.
For control cells, empty vectors without Gateway recombination cassettes were used.
For each plate, we normalized the reduction rates of the treated cells to control cells (empty liposomes or media alone).
Interestingly, expression of Akt pS473 was increased in metastatic AC2M2 cells (empty vector) compared with the poorly metastatic parental SP1 cells.
Identical numbers of parental cells, empty vector and PRDX6-transfected cells were seeded in six-well tissue-culture plates to form colonies.
Even at low power magnification, the morphology of control cells (empty vector), PXN overexpressing wild-type (WT), and mutant cells was dramatically different.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com