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HEK293 cells were washed once with calcium-free PBS and dissociated from flasks with cell dissociation buffer (Invitrogen).
cES were dissociated from a 24 well plate using cell dissociation buffer (Invitrogen).
For epiblast type cells (B D), cells were dispersed into small cell aggregates by Gentle Cell Dissociation Reagent.
G.M.G. performed the organoid cell dissociation and provided quality control for the single-cell preparation, and L.M.D.W. analyzed the scRNA-seq data.
Furthermore, even for tissues that can be available directly from biopsy, the stress of whole cell dissociation may itself influence the expression of certain genes24.
The 10% Ficoll 70 was also able to provide effective cryopreservation for human ESC (H1 cells) that had been dispersed by Gentle Cell Dissociation Reagent into small clumps (Fig. 3C, left panel).
When H1 hESC were dispersed by Gentle Cell Dissociation Reagent, the sizes of the colonies recovered from the Ficoll-containing medium after −80 °C storage were smaller than those of the LN2 controls (Fig. 3C, right panel).
The cell dissociation constant, k(off), increased when the shear stress was elevated at constant shear rate.
Cell dissociation was performed enzymatically [7], [11] or mechanically.
Similar(2)
Y-27632 treatment alone is sufficient to suppress cell dissociation-induced activation of PTEN activity.
We have previously established antibody saturation, monomeric antibody binding, and cell dissociation-receptor effects.
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