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For the SNU alpha particle irradiator, an irradiation system is equipped with cell dishes of 4 µm thick Mylar bottom and a special setup of cells on slide for gamma-H2AX assay.
Two cell dishes have been specially designed: one a Si3N4 plate (2.5 mm × 2.5 mm area with 1 μm thickness) supported by a 7.5 mm × 7.5 mm frame of 200 μm thickness, and the other a Mylar film stretched by pressing with a metal ring.
After 3 h of infection, cell dishes were chilled on ice and rinsed twice with cold PBS.
Preparation of extracts from mouse tissues or whole cell dishes, electrophoresis, blotting, visualization and stripping of membranes were performed as described [84].
Coverslips in live cell dishes (MatTek, MA, USA) or in 6- or 24-well plates were seeded with ∼3×105 cells/ml media, and cells were allowed to adhere overnight.
HepG2.2.15 cells were grown on 10-cm cell dishes.
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(B) Schematic diagram of a 2D culture: HLFs attached to the cell dish bottom.
The uniformity of dose among cells in a dish is achieved by keeping the STD and the size of cell dish in certain ranges.
Those are precise position control of a cell dish holder, design of the cell dish, data acquisition of the microscopic image of a cell organelle (cell nucleus), data processing, reliable particle detection, software and hardware to integrate all these related data, and a system to control and irradiate a targeted spot with an exactly determined number of particles.
Those are precise position control of a cell dish holder, design of the cell dish, data acquisition of microscopic image of a cell organelle (cell nucleus), data processing, reliable particle detection, soft and hard wares to integrate all these related data and system to control and irradiate a targeted spot with exactly determined number of particles.
The treated cell dish was placed on the stage of a temperature-controlled microscope.
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