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The uniformity of dose among cells in a dish is achieved by keeping the STD and the size of cell dish in certain ranges.
Those are precise position control of a cell dish holder, design of the cell dish, data acquisition of microscopic image of a cell organelle (cell nucleus), data processing, reliable particle detection, soft and hard wares to integrate all these related data and system to control and irradiate a targeted spot with exactly determined number of particles.
Those are precise position control of a cell dish holder, design of the cell dish, data acquisition of the microscopic image of a cell organelle (cell nucleus), data processing, reliable particle detection, software and hardware to integrate all these related data, and a system to control and irradiate a targeted spot with an exactly determined number of particles.
However, the inhomogeneous Am-241 distribution in a disc source did not affect the radial distribution of fluence rate at the inner bottom of cell dish when the dish is apart from the source sufficiently.
Dose calibration for the alpha particle irradiator was performed by dual approaches, detection and computer simulation, in consideration of the source-to-target distance (STD) and the size of a cell dish.
During the selection, the size of the positive cell dish was changed to 60 mm ×15 mm, but that of the control cell line was maintained.
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After 3 h of infection, cell dishes were chilled on ice and rinsed twice with cold PBS.
HepG2.2.15 cells were grown on 10-cm cell dishes.
The cells dishes were positioned on this phantom with 2 cm of a water-equivalent phantom above.
Hela cells were seeded in dishes at a density of 5 × 105 cell per dish and the dishes were kept at 37 °C with 5% CO2 for 24 h.
C. Scrape cells off dish with cell scraper.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com