Sentence examples for cell disaggregated from inspiring English sources
Proteases were then inactivated with 10% fetal bovine serum and 2 mM EDTA and the cell disaggregated by passing them through a 40 μm nylon sieve (BD Bioscience).
Following nuclear fragmentation, the cell disaggregates into a number of membrane-bound apoptotic bodies, which are engulfed via phagocytosis by neighboring epithelial cells or macrophages.
In pancreatic ductal adenocarcinoma, RNA-seq has been used to compare genome-wide expression profiles of single cells disaggregated from the primary carcinoma with corresponding CTCs in a mouse model of pancreatic cancer [78].
Mice were killed 3 days post treatment, and tumour masses were resected and cells disaggregated before assessing HLA1 expression as described previously.
As shown in Figure 5, FACS analysis revealed that >30% of the cells disaggregated from the AA rat synovial tissue were P-Alexa-positive.
By BH3-profiling, we confirmed that the cells disaggregated from the spheroids had acquired sensitivity to ABT-737 and to peptides that displace Bim from Bcl-2 and Bcl-XL.
We have carried out a series of experiments to compare the response to radiation and drugs of cells disaggregated from solid tumours as assayed by clonogenic survival and by an isotope incorporation method.
To assess the role of Oct4 in initiating lineage-specific molecular programmes, single-cell transcriptional profiling was performed on individual ICM cells disaggregated from IOD embryos flushed from oviducts at the 8-cell stage and cultured for 24 hours, with or without 4-OHT.
EMT is a process in which epithelial cells disaggregate and exhibit dramatic shape changes.
Primary tumorspheres generated by the CD24+ cells were disaggregated into single-cell suspension; these cells were able to form secondary tumorspheres, which indicates their self-renewal ability (data not shown).
After the cultured-cells reached confluence, cells were disaggregated by trypsinization, three-fold diluted with culture media containing anti-cancer drugs, and then re-plated to a new culture dish.
