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Depending on the spatial and temporal resolution of the imaging techniques, which in typical experiments can range from days down to milliseconds when rapid time-lapse microscopy is utilized, cell diffusion constants can be approximated by tracking the movement of individual cells or analyzing border properties of the cell population (Block et al. 2007).
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The latter mimics cell micro-motility and is quantified by the cell diffusion constant.
These methods allow the deduction of the effective diffusion constants between cells inside the same symplastic domain and the plasmodesma permeability; however it is not possible to distinguish between water flow and solute diffusion.
Simulations of social interactions in spatially structured populations have commonly utilized continuous partial differential equations to model both cell movement and diffusible molecules, with associated bulk diffusion constants [ 16, 18, 22, 24, 25].
The underlying physical parameters are simply the volume of the cell, and the characteristic lengths and diffusion constants of ribosomes and tRNA molecules.
While we can observe FRAP dynamics in differently shaped ERs, we are never sure whether the observed differences in recovery dynamics come from geometric differences or from differences in the molecular diffusion constants in the different cells.
This concentration of Ca2+ may well represent a lower limit since the diffusion constants of buffered Ca2+ in small cells may be lower than in the larger oocytes which we used here.
Rates of initiation and elongation are based on physical parameters that have been experimentally determined in yeast, including the cell volume, the abundances of ribosomes and tRNAs, and their diffusion constants (Tables 1 and S1 available online).
They also diffuse as the grey level with respectively the diffusion constants Lb and Lg.
They noticed that the design of the MSAC network is limited by physical constraints imposed by realistic diffusion constants and the relevant spatial and temporal dimensions in the yeast cell.
Experimentally measured diffusion constants for mRNA transcripts of sizes similar to oskar mRNA (2.9 kB) in mammalian cells are in the range of D = 0.02 μm/s or lower (Mor et al., 2010).
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