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The task of microscopy cell detection is of great biological and clinical importance.
A linear range for the cancer cell detection is obtained between 50 and 100,000 cells/mL with the detection limit of 23±2 cells/mL.
Generally, cell detection is based on indirect methods to collect certain components in a large amount of cells [1 3].
Although TRA does not evaluate the segmentation accuracy, reliable cell detection is the key to this measurement.
Another approach to improving the sensitivity of cell detection is to use polymerase chain reaction (PCR) methods to amplify DNA specific for cancer cells.
Because of the low tumor cell burden in PBL and BM, the accuracy of tumor cell detection is greatly affected by the gene background expression levels.
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The sensitivity of polymerase chain reaction (PCR) in cell detection was negatively affected by some magnetic carriers and compounds used in their preparation.
The PCR sensitivity of target cell detection was negatively influenced by the presence of some compounds used in the process of particle preparation.
In these experiments, the same MNC samples used for CD34+ cell detection were subjected to CFU assay before and after freeze-thawing or freeze-drying and rehydration.
However, the reproducibility of tumour cell detection was reduced after isolation of the mononuclear cell fraction.
Cell detection was performed by manual screening with an optical microscope.
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