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Cells were subsequently stained for cell surface ICAM-1, apoptosis (TUNEL), cell delineation (HCS CellMask), and DNA (DAPI).
Cells were quadruple stained for ICAM-1, apoptosis (TUNEL), cell delineation (HCS cell mask), and DNA (DAPI).
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However, the mechanism of cadherins regulation on endothelial cells requires further delineation.
Nucleoids did appear to be less well defined in PDR1 -249 cells, with reduced delineation between mtDNA puncta; however, the etiology of any minor effect on internucleoid distance induced by PDR activation remains to be determined.
However, the validation of phenomena observed in cells and the delineation of novel functions in more complex tissues is a critical step for accurately characterizing endogenous biological processes, and for defining genomic anomalies in diseased tissues.
A quadruple fluorescent staining was performed using TUNEL (rhodamine) for detection of apoptotic cells, DAPI for the nuclei, high content screening (HCS) CellMask deep red for the delineation of cells, and FITC-labeled anti ICAM-1 antibody for identification of cell surface expression of ICAM-1.
Indeed, monolayer culture of primary hepatocytes offers an astonishing view on cell plasticity, and allows delineation of pathways regulating hepatocyte polarity and homeostasis [ 3].
Here, we have investigated the properties of cells in the macaque LGN using a battery of functional tests including cone-isolating stimuli, and performed histological reconstruction of cell locations with explicit delineation of the koniocellular regions of the LGN.
The resulting mixture was centrifuged at 2800 rpm for 4 min to obtain the PBL, which were washed in 1 % BSA-PBS and used for analysis of the cell surface markers and delineation of the different subpopulations.
Double fluorescent staining was performed using HCS CellMask deep red for the delineation of cells and FITC-labeled anti ICAM-1 antibody for identification of cell surface expression of ICAM-1.
This study contributes to the delineation of cell-nanotopography interactions and provides the insights into the design of nanotopography configuration for pluripotent stem cell neural lineage commitment.
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