Cell determination, data collection, data reduction, cell refinement and absorption correction: CrystalClear-SM Expert 2.0 r7.
Two stations were set up outside and inside the test cell for data collection.
We reviewed the early 'site and services/core house' projects of the 70s/80s, followed by regional presentations by universities who have carried out surveys following our model from our website, and presented innovative new approaches using cell phone data collection and computer modeling.
For cortex cell length data collection, the thick roots can be cleared.
DML participated in experiment design, experiment execution (site density measurements, SQUID measurements, and cell culture), data collection and interpretation, and the writing of the manuscript.
Tao Peng and Xu-Hong Zhou carried out the study design and paper drafting; Min Hu, Ting-TingWu, Cen Zhang, Zhe Chen, and Shuo Huang carried out cell experiment, data collection and analysis, and paper submission.
To exclude signals originating from the flocculated cells, a data collection gate was drawn according to FSC-SSC dot plots from the control strains (Additional file 1: Fig. S3).
After that time, a period established to achieve significant death but leaving a reasonable number of surviving cells for adequate data collection, cells were washed with recording medium and cover slips were assembled into a chamber for confocal imaging.
Tumour tissues fixed in cold 70% ethanol were prepared and analysed using a FACSCalibur (BD Biosciences, San Jose, CA, USA), collecting 15 000 events excluding cell debris from data collection.
