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Only for the most active compounds, further cell cycle tests on HeLa displayed a cell arrest on S phase.
DLMG carried out all the experiments, designed the viability and flow cytometric cell cycle tests and drafted the manuscript.
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The apoptosis test and cell cycle test analysis revealed that etoposide entrapped in calcium carbonate nanobelts (CCNBs) could enhance the delivery efficiencies of drug and improved inhibition effect.
This includes the so-called 'mosaic' patients presenting with normal blood counts and a negative chromosomal breakage or cell cycle test.
A concomitant physical interaction was found to be present throughout the different phases of the cell cycle tested.
Immunoprecipitations with anti-RAN antibody followed by western blotting and detection with anti-MAD2B antibody revealed co-immunoprecipitation during all phases of the cell cycle tested (Fig. 4B; IP).
Cancer cells (2 × 10 cells) were seeded into a 100-mm dish with SN38 or SU11274 (1 μ M) for the cell cycle test.
The proliferation of cBMSCs seeded on the pure CSH and Mg/CSH composite cements was evaluated by using cell cycle test based on flow cytometry.
Based on the effect of IFNA2c treatment on the cell cycle, tested cell lines can be divided into three groups: (1) Daudi, T98G and MDA-MB-231 (increase in G1 fraction, decrease in S and/or G2+M fractions); (2) HeLa S3, B-JAB, and A549 (decrease in G1 fraction, increase in S and/or G2+M fractions); (3) U937 (no effect on cell cycle).
Cancer cells (2 × 10 cells) were seeded into a 100-mm dish with vehicle or EGFR TKI for cell-cycle test.
Statistical significance of differences among proportions of cells in specific phases of the cell cycle was tested by ANOVA followed by Tukey's Multiple Comparison post-test (p < 0.05).
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