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For example, the cell cycle term was enriched amongst genes with increased expression in p27Kip1 null tumors, but it was also enriched amongst genes decreased in Xpcl1+ tumors (Figure 3B,C and Table S2).
Query #5 Compare the expression level of the genes annotated with GO cell cycle term in different breast cancer experiments.
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The cell cycle terms in the second row of Equation (4) implement the continuous cell cycle model of León et al. [50] without G0 phase arrest.
Cell cycle terms were still associated with disease cluster C, albeit with lower ranks than in the BKL analysis.
Nearly all of the DEGs that annotated to cell cycle terms exhibited decreasing mRNA abundances during larval development in both species, as did six of seven annotated transcription factors.
Meanwhile, chromosome organization and cell cycle terms were enriched in down-regulated gene clusters at 4 h in the 1-min NTP-exposed group (upper panel in Fig. 3).
In contrast, genes involved in cancer show different patterns of enrichment, depending upon whether they are somatically mutated, over-expressed or under-expressed: cancer mutated genes show a significant involvement in signaling and apoptosis (e.g., intracellular signal transduction and regulation of apoptosis), while genes significantly over-expressed in cancers are enriched in cell cycle terms.
As was observed for the shared gene list, the cell cycle GO term was significantly enriched and these genes showed decreasing mRNA abundances during larval development.
The network was reconstructed using the 27 genes belonging to the cell cycle GO term and their first and second interaction neighbors obtained from HPRD, BIND, and BioGRID interaction databases.
Cluster 14 contains many genes for proteins known to be involved in the cell cycle (GO term enrichment analysis of this cluster returned P-values of 5.2 × 10-60 for 'cell cycle' and 2.9 × 10-51 for 'mitosis') and supports the involvement of other cluster 14 genes in this pathway.
The type 1 pericyte/FAPs surface marker PDGFRA (CD104A) [ 28, 33] was located in the same cluster as the "cell cycle" GO term in three of the four analyses, suggesting that the FAPs lineage (includes type 1 pericytes and FAPs [ 28]) was a major contributor to the cell cycle gene expression signal.
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