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Recent evidence showed that the nucleus of ESCs, fetal fibroblast or cumulus cells could be reprogrammed by the cytoplasm of interphase 2-cell embryo blastomeres after cell cycle synchronization, however, the interphase 2-cell embryo cytoplasm only could support the cell cycle synchronized ESC nuclear transfer embryo development to term (Kang et al., 2014).
MutuI cells were fractionated by centrifugal elutriation to isolated cell cycle synchronized populations.
After 24 h, cells were cell cycle synchronized in 100 μl culture medium containing 0.2% FBS for additional 24 h.
This dataset contains microarray gene expression measurements at 24 time points of the yeast cell cycle synchronized by cdc15.
This change in gene expression was observable in the condition-specific LNS analysis of cell cycle synchronized cells because this alternate phase of expression changed the correlations with other periodically regulated genes.
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Four treatment groups were analyzed: one received control diluents ("vehicle") but no cell cycle synchronizing drug; a second received nocodazole synchronization only; a third received lovastatin only; and a fourth received nocodazole+lovastatin (Figure 4A).
Cell cycle was synchronized by serum starvation for 24 hours followed by refeeding.
Therefore, in this paper we focus our analysis on the microarray cell cycle data synchronized by α-factor arrest.
We used S. cerevisiae cell cycle data synchronized with CDC28 [ 34], CDC15 [ 33] and α-factor [ 33].
Here, we further analyzed the temporal expression of CDKA1 and CDKA2 during the cell cycle in synchronized P. tricornutum cells in the absence of nocodazole.
To investigate whether PLK1 regulates NUAK1 expression in the cell cycle, we synchronized U2OS cells in the G2 stage of the cell cycle using the DTB and CDK1 inhibitor (RO3306) release protocol [ 39].
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