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It is assumed that cells grow in a continuous bioreactor, in two cell cycle stages.
BMP2-iPSC-MSCs did not compromise growth kinetics and cell cycle stages compared to iPSC-MSCs.
Adjusting distinct growth rates in chemostats with the model strain Pseudomonas putida KT2440, cells were differentiated by DNA content reflecting different cell cycle stages.
Patient-derived xenograft BCP-ALL cells were sorted according to cell cycle stages (i.e. G0/G1 and G2/M) and subsequently transplanted onto NOD/SCID mice.
However, the cells treated with the inhibitor MLN8237 at earlier cell cycle stages (wherein the cells remained undivided) had a normal Golgi architecture.
Then the cells were harvested, sorted into cell cycle stages and used for chromatin immunoprecipitation experiments.
The cell cycle stages were analyzed using the FACS Calibur (Becton & Dickinson, NJ, USA).
During these time intervals, cell cycle stages were determined by FACS analysis as reported before [17], [32], [33].
Therefore, we determined the c-Myb protein levels that were associated with chromatin during the different cell cycle stages.
In a log population, cells are present at all cell cycle stages, including some within a few hours of S-phase and others in early G1.
Nevertheless, all these studies demonstrate quite variable stability of H3K27me3 on the marsupial Xi in time (cell cycle stages) and space (cell types, species).
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