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Cell cycle distributions after irradiation were assayed with flow cytometric analysis.
A probabilistic model for cell cycle distributions in synchrony experiments.
We demonstrate that the model reliably fits data collected from populations synchronized by multiple techniques, and can accurately predict cell cycle distributions as measured by other experimental assays.
We next investigated the 'snapshot' cell cycle distributions of low- and high-biotin cells.
Cell cycle distributions were modeled using Multicycle (Phoenix Flow Systems, San Diego, CA).
While similar cell cycle distributions were observed in unirradiated Rad21+/− and WT cells, the two cell types exhibited different profiles when examined 24 hours post IR (Fig. 2A).
The percentages of cell cycle distributions were transformed by arcsine of the square root prior to ANOVA analysis followed by Tukey's test.
To assay chromosomal instability, sorted diploid populations of U87MG and U87MG-EGFRvIII were passaged every 3 days, and cell cycle distributions were analyzed by flow cytometry after propidium iodide staining [33].
To determine whether the reduction in the number of prostate cancer cells upon USP19 depletion resulted from a delay in cell cycle progression, we measured the cell cycle distributions of DU145 cells transfected with control or USP19 siRNAs.
Cell cycle distributions were analyzed with FACSCalibur and CellQuest software.
Cell cycle distributions were analyzed by DAPI staining.
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