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Comparison of GO biological enrichment pinpointed regulation of genes involved in cell cycle, developmental pathways, lipid metabolism, and protein deubiquitination.
These data confirm that Ring1b is involved in regulating cell cycle, developmental and chromatin-related processes and indicate that deletion of Ring1b affects glyconeogenesis and the regulation of cellular physiological processes.
In summary, progressive changes in regulation of cell cycle, developmental and lipid metabolic functions chaperoned EGF-induced hepatocarcinogenesis, whereas potential switch-like regulation was observed for small groups of genes defined by protein deubiquitination and bile acid biosynthesis, a component of hepatic cholesterol metabolism.
All 4 pathways are central in developmental and stem cell biology where they control cell cycle, developmental potential, cell adhesiveness and apoptosis [ 8, 10].
We selected genes for Q-PCR on the basis of the magnitude and direction of gene expression from each of the categories in Table 2, namely, signal transduction, apoptosis, cell proliferation and differentiation, cell cycle, developmental process, immunity and defense, and intra-cellular protein traffic.
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Furthermore, facile in vitro fertilization provides superbly synchronized embryos for cell cycle and developmental stages.
Different cell cycle and developmental stages impose specific shapes on the microtubule cytoskeleton.
C. crescentus swarmer cells can be isolated in a density gradient and these cells will pass synchronously through the cell cycle and developmental program [10].
In contrast to cell cycle and developmental categories whose enrichment in both transgenic and tumor gene sets reflects progressive regulatory alterations during carcinogenesis, downregulation of genes associated with protein deubiquitination, a relatively new target for cancer therapy, was first observed in tumor cells.
The basolateral localization appears to be independent of the cell cycle and developmental stages.
Cell cycle and developmental regulator genes are another class that undergo changes in expression.
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