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Whether CICs undergo similar cell cycle changes in vivo upon radiation and whether cycling CICs become susceptible to drugs targeting cells moving through G2 phase remain to be determined.
A remarkable example is the viral induction of cell cycle changes in the photosynthetic picoeukaryote Emiliania huxleyi.
Since cells cannot be synchronized in vivo due to constitutive expression of PyV MT, it is difficult to evaluate cell cycle changes in tumors.
Utilizing a thymidine-synchronization model, we have analyzed the cell cycle changes in the profiles of tachyzoite mRNAs that accompany nearly two cycles of tachyzoite division.
Reincke et al [21] also found this cell line difficult to transduce with recombinant adenovirus containing SMARCB1 and reportedly found inconsistent cell cycle changes following infection.
Exogenous oleic acid markedly reversed the cell cycle changes produced by SCD1 inhibition demonstrating the critical importance of MUFA to cell cycle progression.
To quantify cell cycle changes in cell volume, we compared the mean nuclear diameter of cells with a single nucleus before mitosis (di) (Fig. 1, b c) and in cells with two nuclei after (df) mitosis (Fig 1g) (Table S1).
To assess the stage of functional differentiation of mitochondria during the cell cycle, changes in the mitochondrial membrane potential (ΔΨm) were determined by FACS analysis using the TMRM+ probe.
Our results identify cell cycle changes in gene expression in response to carbon starvation that involve the prominent role of the FixK FNR/CAP family transcription factor and the CtrA cell cycle regulator.
Cell cycle changes were detected by flow cytometric analysis.
Cell cycle changes were investigated using flow cytometry.
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