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FACS analysis for cell cycle assessment was performed with Propidium Iodide staining five days after transfection as described previously [ 52].
In conclusion, the immunohistochemical method for cell cycle assessment that we describe offers numerous practical benefits, in that it is reproducible, can be standardised in any diagnostic histopathology laboratory and the analysis can be confined to the neoplastic component.
Analysis of cell proliferation applying antibodies specifically detecting members of the MCM protein family has been proposed as a novel method for cell cycle assessment which may be of diagnostic and prognostic value in the histopathologic assessment of neoplasms.
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Various proliferation markers (e.g., Ki67 shown in Figure 2A, BrdU, PH3, and Aurora B), are widely used for cell-cycle assessment and quantification of cell proliferation; however, they sometimes prove insufficient, especially in CMs in which polyploidization and binucleation are natural outcomes of cell proliferation in the postnatal period (Bersell et al., 2009; Zebrowski and Engel, 2013).
Pluripotent cells can be split after EdU treatment into two tubes, one for EdU assessment of cell cycle profile and the other for assessment of apoptosis by DiLC (Mitochondrial membrane potential).
Two illustrations are presented, one covering an efficiency assessment of a PEM fuel cell, and the other a life cycle assessment of fuel cell vehicles.
This present paper aims to trace the progress of dye sensitized solar cells from a life cycle assessment perspective in order to draw the environmental profile of this technology for assessing its potential for eco-friendly innovation in the energy sector.
One half was fixed in 10% neutral formalin for histomorphology, BrdU, and apoptosis assay; the other half was frozen in liquid nitrogen and stored at -80°C for the assessment of senescence-like cells by SA-β-Gal staining, for the assessment of cell cycle distribution by flow cytometry, and for western blotting.
Assessment of cell cycle status by flow cytometry 12 hours after treatment showed that -JQ1 induced arrest in G1 (Fig. 3f,g), consistent with previous reports14, 15, 22, 38.
Other methods for assessment of cell cycle have been published before.
Cell cycle analysis was performed by assessment of the DNA content using propidium iodide.
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