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The in vitro effects of ZOL were studied by proliferation, apoptosis, and cell cycle analyses on the osteosarcoma cells OSRGA compared to rat primary osteoblasts.
Using SYTOX Green instead of propidium iodide (PI) in a standard ethanol fixation protocol increases the accuracy and reproducibility of cell cycle analyses.
For an improved understanding of these effects, this study performed further cell cycle analyses of 7, 13 15, and sunitinib (Fig. 3).
We performed cell cycle analyses using propidium iodide and BrdU to label proliferating cells (Figure 1E).
Cell cycle analyses used propidium iodide (Sigma) in a modified Vindelov's preparation.
Cell cycle analyses was performed with nuclei isolated from MSC-derived myotubes.
Cell cycle analyses were done using flow cytometry using a FACS calibur flow cytometer [50].
Cell cycle analyses at day 7 identified a marked G1 cell cycle arrest in those cell lines that displayed differentiation in response to B/M (data not shown).
Incorporation of EdU, a BrdU-like marker for newly synthesized DNA also allows for the cell cycle analyses in unsynchronized cell populations.
Cell cycle analyses of NPCs revealed that IFNγ decreased the S-phase together with an increase in the sub-G0 phase (data not shown).
Infections were performed at a multiplicity of infection (MOI) of 1.0 for cell cycle analyses as shown in Table 1 and Fig. 1.
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