Sentence examples for cell culturing protocols from inspiring English sources

The cell Culturing protocols have been previously described [19].

The cell culturing protocol was carried out in accordance with the Animal Health Laboratories procedure VIW-17 using a Cell Growth Medium 199 (Sigma-Aldrich) and 10% fetal calf serum (FCS) (Virology Laboratory procedure VIW-17, Animal Health Laboratories, Animal Virology, Department of Agriculture and Food, 3 Baron Hay Court, Kensington, Western Australia, Australia).

We cultured the explants following standard cell culture protocols.

After amniotic fluid isolation, cells were immediately cultured, maintained and passaged in vitro following standard cell culture protocols.

Further information on cell culture protocols can be found in the Additional file 1.

For endothelial cell culture, protocols were adapted based on the recommendations for microcarrier culture using Cytodex-3 (GE Healthcare, Piscataway, USA).

Knowledge of the mechanisms that regulate hepatic epithelial cell differentiation has been essential in creating efficient cell culture protocols for programmed differentiation of stem cells to hepatocytes as well as developing cell transplantation therapies.

More specifically, we discuss developments in biointerface science that are an important driving force for new biomedical materials and advances in bioengineering aiming at improving stem cell culture protocols and 3D scaffolds for clinical applications.

This whole workflow is non-invasive, compatible with many cell culture protocols, technically straightforward and rapid, particularly regarding mass spectrometer time usage and could make label-free LC-MALDI analysis of low-complexity proteomes a major tool for routine cell culture characterization.

Tumor cell lines and cell culture protocols were previously described [7].

A better understanding of such adaptation processes will be essential for the optimization of stem cell culture protocols and the design of injectable biomaterials [22], [63], [64].