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In vitro cell cultivation experiments with MC3T3-E1 preosteoblasts were performed on NF scaffolds and on similarly designed solid-walled (SW) scaffolds that did not have nano-fibers.
AK, AP, and CK performed the Spirulina cell cultivation experiments.
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High cell density cultivation experiments were carried out as fed-batch fermentations using dual control algorithms of carbon source feeding.
YLZ participated in the design of cell purification and cultivation experiments and manuscript preparation.
LS was responsible for cell cultivation, transfection, FRAP experiments, statistics, and regression analysis, and writing the manuscript.
The cell cultivation procedure for the following experiments is described in the Additional file 1. Polar and apolar lipid extracts were prepared from the dried cell pellets using established procedures [ 49].
Katrin Darm contributed to cell cultivation, plasma treatment, and DIGE experiment.
Various cultivation experiments at the 30-L scale revealed very low cyanophycin contents of the cells ranging from 4.6%to6.2%2% (w/w) of cellular dry weight (CDW) only, most probably because most cells had lost the corresponding plasmid during cultivation.
Cell cultivation robot from Kawasaki Heavy Industries.
A variety of bioreactors for cell cultivation exist and can be readily adapted for stem cell cultivation and differentiation.
Three independent cultivation experiments were carried out for shake flask experiments and two in case of bioreactor experiments.
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