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Few high-throughput methodologies exist that measure cell compliance, which can be used to understand the impact of genetic alterations or to screen the efficacy of chemotherapeutic agents.
The mechanical properties of the cell were determined through AFM indentation measurements of cell compliance.
Knockdown of CD44 increases cell compliance, enhances migration potential, facilitates tumor growth and promotes lung metastasis [ 15].
The AFM is a versatile tool that can be used to study cell adhesion as well as cell compliance.
Cell compliance studies can complement the adhesion studies with information concerning the mechanical properties of the cell.
We describe the use of atomic force microscopy (AFM) in studies of cell adhesion and cell compliance.
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Before drinking my first cup of coffee, I like to get my cell in compliance.
The deformation of soft muscle cells in compliance of blood vessel wall is crucial for hypertension.
Linear static analysis performed on the perfect geometry under own weight showed an expected prevailing compression regime: higher axial stresses were in areas close to the base constraints (Fig. 4a), where the Voronoi pattern has more stretched cells, in compliance with the property of stress field anisotropy (Pietroni et al. 2015).
Further verification of the protein expression level of S100A2 by Western blot analysis using an antibody specific to S100A2 confirmed that the expression of S100A2 in KKU-M213 is obviously higher than KKU-100 cells), in compliance with the expression profile of proteomic analysis.
In addition, molecular docking, cell cycle analysis, compliance to Lipinski's rule of five, and in silico toxicity assessment have been reported.
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