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"Microfluidic transport in microdevices for rare cell capture". Electrophoresis 33(21): 3333-3342.
Cell capture experiments demonstrated that cell immobilization was optimized throughout the whole flow chamber.
Contrary, cell capture on mineralized PLGA/Col was comparable to pure PLGA/Col.
The cell capture structures and filters, fabricated on PDMS (polydimethylsiloxane), included two structural layers.
Initial cell adhesion was evaluated by cell capture efficiency based on the cell count method.
Here, we have developed a method to capture select microorganisms directly from the environment, using combined fluorescence in situ hybridization and immunomagnetic cell capture.
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Sequential rounds of cell capture-wash-release-recapture result in an enrichment of epithelial cells by 108-fold.
An efficient single-cell capture in microwells is crucial for fluorescent identification.
The whole process, including a single-cell capture and Raman measurement, was performed within one second.
Single-cell capture pools were sequenced with an Illumina MiSeq to verify successful L1-Ta enrichment (data not shown).
Consequently, we developed a new cold fixation protocol (70% ethanol fixation at -10°C), allowing efficient single-cell capture.
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