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After completion of the RA procedure, reception of data from the small cell buffer can start.
Since the data to transmit needs to be transferred from the macro cell buffer to the small cell buffer during this procedure, data reception from the macro cell is unavailable during the whole RA procedure time.
Averaging NPP for a three cell by three cell buffer around the flux tower location resulted in a slight decrease in correlation between modeled NPP and flux tower measurements (R2 = 0.71).
The proposed priority traffic regulator maintains a dedicated cell buffer for each type of traffic to be able to trade off cell delay and loss (or tagging) on the basis of the QoS requirements for each priority class.
This suspension was incubated at 37°C for 1 h with gentle shaking and then washed three times with 10 volumes of BSGC buffer and centrifuged at 1500 g for 5 min. This sequence of washes of the cells is necessary to assure that neuraminidase is excluded from the cell buffer suspensions and subsequent experiments [5], [12].
Resuspend in 1 ml of buccal cell buffer.
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Cells were lysed in a whole-cell buffer containing protease and phosphatase (10 mM NaF, 10 mM Na-pyrophosphate, 1 mM Na3VO4) inhibitors, then the supernatants were obtained using centrifugation at 4 °C, 14 000 r.p.m. for 15 min.
After isolation, mitochondria were suspended in isolation buffer for cells (buffer C) and kept on ice.
In the second strategy, reservoir cells and buffer cells are used, and this is the approach adopted in our general hybrid scheme.
Nicotine- or buffer-treated cells were lysed on ice at the various time points with Cell Signaling Technologies cell lysis buffer.
Carefully resuspend the cells in cell binding buffer to a concentration of 2 × 106 cells/mL.
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