To detect ubiquitinated TIP60 proteins, cells in a 10 mL cell bottle were transiently transfected with 4 μg HA-ubiquitin-expressing plasmids together with the indicated plasmid.
Interesting as this view is, I reject it because, with Dawkins (1982), I believe that reproduction, in particular going through a single cell bottle neck from whence the developmental process is started anew, is necessary for the evolutionary process of adaptation.
Medium in cell culture bottles was changed after 48 h and non-adherent cells were removed; then, culture medium was changed once every 48 h.
The IEC-6 cell culture bottles were fixed on a plastic disc.
After another washing step, 2.5 × 105 cells were seeded and expanded in passage 1 into T75 cell culture bottles.
The microcosms were filter-capped 250 ml cell culture bottles (Corning) containing 150 ml of the medium.
At the day of inoculation, two cell culture bottles with massive growth of L. intracellularis were used.
After incubation, collagenase solution containing endothelial cells was eluted from the umbilical cord, cells were washed by centrifugation in PBS and plated in T25 cell culture bottles (Cell+, Greiner, Frickenhausen, Germany) in Endothelial Cell Culture Medium (Promocell, Heidelberg, Germany).
When the IEC-6 cell culture bottles were radiated by neutron, the plastic disc was rotating (rotation speed of 10 cycles per minute) in order to assure the IEC-6 cells received even neutron irradiation.
To screen for WNV, cell culture bottles showing viral CPE involving at least 75% of the Vero monolayer were harvested after one freeze-and-thaw cycle and tested for WNV by reverse transcriptase polymerase chain reaction (RT-PCR) (8 ).
