These small, high density cells were additionally distinguishable from the surrounding and larger epithelial cells based expression of pcp, a pupal cuticle gene; only the larger cells expressed pcp. Mitotic activity did not initiate until about 20 24 hrs a.p. and then only at a low level, increasing significantly around 40 48 hrs a.p. and continuing to around 72 80 hrs a.p.
Thus, in order to produce readily high amounts of recombinant VGLUT1, the powerful Baculovirus expression system was attempted as an alternative to the PC12 cell based VGLUT1 expression.
In agreement with our cell based studies, PAK6 expression was lower in prostate cancer (p = 0.003, fold change = -1.350).
Thus, a method to estimate relative transcriptome sizes is needed for determining expression differences per cell based on transcriptome-normalized expression profiling data.
Sorting ObL cells based on expression of ALP, a well-established osteoblast marker, allows enrichment for immature versus more mature osteoblastic precursors [23].
Strategies for enrichment have included manual dissection of beating areas [10], [11], Percoll® density gradient sedimentation [7], [12], and fluorescence activated cell sorting (FACS) of cells based on expression of a fluorescent reporter protein from cardiomyocyte gene promoters [11], [13].
We sorted spheroid cells based on expression of CD44/CD54 and then examined stem cell markers among the different cellular subpopulations (CD44+CD54+, CD44+CD54−, CD44−CD54+, CD44−CD54−) derived from rectospheres.
Some studies have also used a new CellSearch System technology that employs immunomagnetic separation of epithelial cells based upon expression of cytokeratins or EpCAM and visualization of the tumor cells by immunoflorescent microscopy [ 30].
The activated microglia cells based on expression of Iba-1 on the ipsi-lateral side of the spinal cord increased significantly at early (p < 0.05), but had no change at the late stage as shown in Fig. 5.
Issues concerning in vivo applications, such as cell tracking in deep tissues, are different from that concerning analysis of gene expression in cell based assays.
