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The low cost imaging device compared favorably against the expensive commercial luminometer plate reader using the same cell based assay.
Forty two (42) potential factors were identified using the cell based assay.
Antioxidant activities were assessed using the DPPH, ORAC, FRAP and DCFH-DA (cell based) assay.
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The major concern in using T-cell bassayassay is the influence of CD4 count on the sensitivity.
The experiment was based on a previously described method [ 27] adapted to cell-based assay.
Notably, this assay provided greater sensitivity in detecting seeding by polyQ than the cell-based assay to monitor visible aggregates (Figure 2b).
400 μM tBHP was found to be optimal as it elicited a robust and reproducible stressor effect in this cell-based assay.
(a) Cell-based assay of canonical Wnt pathway activities through a TCF/LEF luciferase reporter system composed of the TOPflash and control FOPflash constructs.
MSH2 promotes CAG repeat contractions together with the NER factor, Xeroderma Pigmentosum, Complementation Group A (XPA 15, in a human cell-based assay.
Bao, Y. et al. A cell-based assay to screen stimulators of the Hippo pathway reveals the inhibitory effect of dobutamine on the YAP-dependent gene transcription.
Regardless of the technology (RNAi, CRISPR, over-expression, etc)., a good cell-based assay is the best foundation for a cell-based screen.
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