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The loads of the specimens were measured by a load cell attached to the actuator.
6 h, 12 h, 24 h after inoculation, the cell attached ratios were calculated respectively.
The axial load was measured by a load cell attached to the lower piston inside the pressure vessel.
Cell attached recordings were done in voltage-clamp mode with loose seals in between 10 - 20 MΩ.
A representative current trace from a cell attached patch showing such activation is reported in Figure 3A.
The cell attached mode of the patch clamp technique allows for stable measurements at the single molecule level.
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The loading value was measured with a load-cell attached to the tensile test machine.
In contrast, cell-attached and inside-out patches required similar pressures for activation with positive pressure (cell-attached: P50 = +19.3 ± 1.2 mmHg; N=12; inside-out: P50 = +26.7 ± 4.2 mmHg; N=7; P>0.05).
Open probabilities (Po) of the BK channel were measured using the cell-attached patch clamp technique.
We also examined spontaneous fast current transients (CTs) recorded from these pyramidal neurons under cell-attached configuration.
Pipettes for cell-attached experiments contained 150 mM NaCl.
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