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Both the number of mitochondrial profiles and mitochondrial mass per cell, assessed by flow cytometry of cells stained with nonyl acridine orange, increased at 24 72 h of treatment with hsp90 inhibitors (Fig. 1 B D and data not shown).
Although these analyses can be applied to a sample of cultured bacteria following the classic dilution and plating based methodology, FCM offers real measurements for each cell assessed, and in less time.
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Tumor cells assessed by MTT assay and LC50 value were found to be 3.5 ng.
Sizes of PC12 cells assessed by our system matched conventional measurements.
These dimensions fit the percentages of CD44s and CD44v6 expressing cells assessed herein.
Our data show that a functional heterogeneity of β-cells, assessed by PSA-NCAM surface expression, exists in vivo.
After 2 weeks of culture, the viability of cells assessed by flow cytometry using 7AAD stain was more than 90%.
The purity of recovered cells assessed by flow cytometry was greater than 95%.
Purity of isolated cells, assessed by flow cytometer, reached routinely at least 97%.
The purity of B and T cells assessed by flow cytometry was >98%.
ELISA data were normalized to total protein concentrations of cells assessed by the Bradford assay [ 14].
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