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We grew a colony of cells in 0xMet, shifted to 10xMet and recorded the division time of each cell (as defined by CDC10-YFP) relative to the media shift, and their subsequent history.
These findings confirm a precancer 'stem' cell, as defined by the AACR working group [ 11], as the origin of invasive cancer.
Until we have this information, it is important to consider independently the concepts of cell of origin and cancer-propagating stem cell, as defined purely by self-renewal and capacity to differentiate.
Thus, this protocol allows us to measure the intensity of GFP within each cell as defined by the mask that is used for the determination of the morphology of that cell [ 27].
Nuclear GFP fluorescence intensity was quantified on a per cell basis from a single optical section (as defined by a binarized mask created from the corresponding DAPI signal), then subtracted from the total somatic integrated intensity for each cell (as defined by a binarized mask created from the corresponding tdtomato signal) to yield the uniquely cytoplasmic intensity signal.
By performing serial dilutions as well as transplanting single marked cells that were confirmed by visual inspection they calculated that at least one out of 64 cells bearing the phenotype Lin-CD29hiCD24+ Lin-CD29hiCD24+ as defised by its ability to reconstemute an entire mammary gland.
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Results Female cells, as defined by the presence of intact nuclei with two X chromosome signals, were detected in multiple tissue types from all 4 male neonates.
We also comment on the connection between the hole size as measured by PALS and the size of a cluster of randomly distributed unoccupied cells as defined by the Simha Somcynsky theory.
As will be detailed in the next sections, the complex cells, as defined in this paper, have DSP kernels that are low-rank and include those shown in Fig. 1 as a particular case.
Introduction: Both anaemia and transfusion of red cells (as defined by WHO criteria) (1) have been associated with adverse outcomes, and the potential for anaemia to be a marker of a greater disease burden is frequently raised in discussion.
The Pre-Pro/Pro B cells, as defined by IgM−B220loCD43+ [21], [21], expressed higher levels of the Siglecg transcripts (Fig. 1B).
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