Stained cells were acquired on a BD LSR Fortessa cell analyzer and analyzed with FlowJo (Treestar) software.
Cells were then analyzed using a LSRFortessa cell analyzer (BD Biosciences), and data analyzed using FlowJo version 7.6.4 software.
The granule intensity in each cell was measured and statistically analyzed by IN Cell Analyzer workstation version 3.2.
Stained cells were analyzed using BD LSRFortessa cell analyzer (BD Bioscience).
The percentage of mCherry-positive cells was analyzed using BD LSRFortessa cell analyzer (BD Biosciences, San Jose, CA, USA).
Samples were analyzed on an Accuri cell analyzer.
All samples (50 000 events/sample) were analyzed on an LSRFortessa cell analyzer (BD Biosciences).
Stained cells were then analyzed using a FACSCalibur cell analyzer (Becton Dickinson).
After 20 min, the percentage of apoptotic cells was analyzed by the Muse™ Cell Analyzer (Millipore).
Flow cytometry was performed on a LSRFortessa cell analyzer (BD Biosciences), and data were analyzed with FlowJo 7.6.4 software (Treestar).
Cells were imaged using the In Cell Analyzer 1000 and pictures were analyzed using the In Cell Investigator 3.4 software.
