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This approach automatically includes all deformation mechanisms as opposed to the classical unit cell analyses of bending beams.
It is also based on single cell analyses of the CD4+ T cells undergoing a primary response where R5 HIV-1 infection and the synthesis of CCL4 were largely exclusive.
The single cell analyses of cdr2-GFP in the wildtype used 13 confocal sections 0.4 µm apart.
Real time cell analyses of proliferation, adhesion and migration were performed in triplicates on xCELLigence instruments (Roche, Hvidovre, Denmark).
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Single-cell analyses of transcript and protein expression profiles — more precisely, single-cell resolution analysis of molecular profiles of cell populations — have now entered the center stage with widespread applications of single-cell qPCR, single-cell RNA-Seq and CyTOF.
These two methods will greatly improve single-cell analyses of fission yeast by flow cytometry and thereby allow a much more detailed, exact and sophisticated cell-cycle analysis.
Our single-cell analyses of Hsp104-GFP partindicatedindicathatthat a relatively minor change in chaperone retention from 65%to75%5%, which corresponded to a 2.2-fold increase in accumulation based on fluorescence intensity (compare 37°C 40°C, Table 1, Figure 5A), correlated with a quantitative switch from prion stability to curing, suggesting the existence of a biological threshold in this range.
The introduction of PTEN to BRCA1 mutation-prediction algorithms is supported by single-cell analyses of temporal somatic events in BRCA1 breast tumor tissue, which revealed that loss of PTEN is an early event in the development of BRCA1 basal-like tumors, whereas TP53 mutations occur first in most luminal BRCA1 tumors [ 12].
The balance of proteins not identified in ovarian cancer cell analyses consisted primarily of inflammatory and immune response related proteins.
This surprising result highlights the power of single-cell analyses to define CN states of somatic mutations.
(D ) Propidium iodide-based cell cycle analyses of cells from zebrafish embryos (24 hr) treated with or without SPOP-specific morpholino.
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